The COVID-19 pandemic has resulted in demand for population scale pathogen screening. The most sensitive and reliable method for detecting SARS-CoV-2 remains reverse transcription PCR (RT-PCR).

Digital PCR, or dPCR, is a molecular biology technique that detects and quantifies nucleic acid targets. By partitioning a sample into many individual reactions, scientists can measure whether a ...

PCR, or Polymerase Chain Reaction, is a revolutionary molecular biology technique that allows for the rapid amplification of specific DNA sequences. It is an essential tool in various fields, ...

During the early stages of the coronavirus disease 2019 (COVID-19), rapid testing was imperative for public health policymakers and health care workers in order to determine where and what measures ...

Human papillomavirus (HPV) is a double-stranded circular DNA virus with a genome of approximately 7–8 kb. This study aimed to ...

Gene-Analysis Studies Advanced by Use of Low-Volume High-Throughput Real-Time PCR This article describes three gene-analysis studies using the Roche LightCycler® 1536 Real-Time PCR System (Figure 1).

Failed polymerase chain reactions (PCRs) are a common cause of frustration in the lab. While a typical PCR protocol is quite straightforward, scientists must optimize each step to amplify the intended ...

Human papillomavirus (HPV) is a double-stranded circular DNA virus with a genome of approximately 7–8 kb. This study aimed to establish an overlapping extension polymerase chain reaction method for ...